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Note: To use pure cultures you must have suitable equipment, some knowledge and skill.

A mycelium pure culture is obtained by germinating spores on a culture medium, in this case the base is agar.
By selecting the right mycelium, one eventually obtains a potent strain, or, productive mycelium.

The pure culture can be delivered in 2 ways.

As slant culture in culture tube:

  • Stable for a few months in the refrigerator (4°C).
  • Less susceptible to contamination (contamination with other organism) due to the narrow opening
  • Intended for further cultivation
  • Requires some skill.
     

As culture in petri dish:

  • Few weeks to keep in refrigerator (4°C).
  • Intended for further cultivation.
  • Very suitable for direct inoculation of sterilized grain.

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